Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: A. Normal bone marrow, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. C. AHSP stains nucleated EPs, but not mature, anucleate RBCs. D. CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ fully automated for IHC.
AHSP / EDRF Antibody (Liquid)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744601-25
Catalog Number: LS-C744601
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- Description
- EDRF antibody LS-C744601 is an unconjugated rabbit polyclonal antibody to EDRF (AHSP) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- EDRF | Erythroid-associated factor | AHSP | ERAF | Erythroid associated factor
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC
- 1:8000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q9NZD4
- NCBI GenBank
- NM_016633 | NP_057717.1
- SwissProt
- Q9NZD4
Target
- Target
- Human AHSP / EDRF
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest cross reactivity with human and mouse. Cross-reactivity with AHSP from other sources have not been determined.
- Gene Name
- AHSP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 115 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl