Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Immunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A. Normal bone marrow, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. C. AHSP stains nucleated EPs, but not mature, anucleate RBCs. D. CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max fully automated for IHC.
Polyclonal Rabbit anti-Human AHSP / EDRF Antibody (Lyophilized, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C153762-100
Catalog Number: LS-C153762
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- Description
- EDRF antibody LS-C153762 is an unconjugated rabbit polyclonal antibody to EDRF (AHSP) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- EDRF | Erythroid-associated factor | AHSP | ERAF | Erythroid associated factor
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC-P
- 1:8000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q9NZD4
- NCBI GenBank
- NM_016633 | NP_057717.1
- SwissProt
- Q9NZD4
Target
- Target
- Human AHSP / EDRF
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest cross reactivity with human and mouse. Cross-reactivity with AHSP from other sources have not been determined.
- Gene Name
- AHSP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 115 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
a-Hemoglobin-stabilizing protein is a sensitive and specific marker of erythroid precursors. Raess PW, Paessler ME, Bagg A, Weiss MJ, Choi JK. The American journal of surgical pathology. 2012;36:1538-47. PubMed: 22982896 PMC: PMC3445287
