CD97 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg SW480 Whole Cell Lysate. 0.5 uL anti-CD97 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa.
ADGRE5 / CD97 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C817880-100
Catalog Number: LS-C817880
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- Description
- CD97 antibody LS-C817880 is an unconjugated rabbit polyclonal antibody to human CD97 (ADGRE5). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD97 | CD97 molecule | Leukocyte antigen CD97 | TM7LN1 | CD97 antigen
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P48960
- NCBI GenBank
- NM_078481 | NP_510966.1
- SwissProt
- P48960
Target
- Target
- Human ADGRE5 / CD97
- Antigen Species
- Human
- Epitope
- Human CD97
- Gene Name
- ADGRE5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human CD97 protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS