Western blots of rat olfactory epithelium (OE) and frontal cortex (FC). The antibody stains bands at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.
ADCY3 / Adenylate Cyclase 3 Antibody (aa1125-1144)
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
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- Description
- Adenylate Cyclase 3 antibody LS-C204505 is an unconjugated rabbit polyclonal antibody to Adenylate Cyclase 3 (ADCY3) (aa1125-1144) from rat. It is reactive with human, mouse and rat. Validated for ICC, IF and WB. Cited in 2 publications.
- Application
- ICC, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Predicted Reactivity
- Horse, Pig, Rabbit
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AC-III | AcIII | Adenylyl cyclase 3 | AC3 | Adenylate cyclase type III | ATP pyrophosphate-lyase 3 | ADCY3 | Adenylate cyclase 3 | Adenylate cyclase type 3 | Adenylyl cyclase, type III | KIAA0511
- Recommended Dilution: IF/ICC
- 1:500 - 1:1000|0.388888888888889 - 1:1000
- Recommended Dilution: WB
- 1:5000 - 1:10000
- UniProt Number
- O60266
- NCBI GenBank
- NM_004036 | NP_004027.2
- SwissProt
- O60266
Target
- Target
- Rat ADCY3 / Adenylate Cyclase 3
- Antigen Species
- Rat
- Epitope
- Mouse and rat Adenylate Cyclase 3.
- Gene Name
- ADCY3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic peptide PAAFPNGSSVTLPHQVVDNP with a cysteine residue added to the N-Terminus to allow coupling to KLH. The peptide corresponds to the extreme C-Terminus of rat molecule. Percent identity by BLAST analysis: Mouse, Rat (100%); Horse, Rabbit (95%); Pig (85%); Human, Chimpanzee, Guinea pig (80%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling. Bandarigoda Nipunika Somatilaka, Sun-Hee Hwang, Vivek Reddy Palicharla, Kevin Andrew White, Hemant Badgandi, John Michael Shelton, Saikat Mukhopadhyay. Developmental cell. 2020 September;54:710-726e8.
PubMed: 32702291 · PMC: PMC9042708
Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E. Skylar I Dewees, Romana Vargová, Katherine R Hardin, Rachel E Turn, Saroja Devi, Joshua Linnert, Uwe Wolfrum, Tamara Caspary, Marek Eliáš, Richard A Kahn. Molecular biology of the cell. 2022 April;33:ar33.
PubMed: 35196065 · PMC: PMC9250359