Western Blot of rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated. Lane 1: Adenosine Deaminase (Calf Spleen). Load: 50 ng per lane. Primary antibody: Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 41 kDa, 45 kDa for Adenosine Deaminase (Calf Spleen).
ADA / Adenosine Deaminase Antibody (Lyophilized, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Bovine |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C745033-100
Catalog Number: LS-C745033
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- Description
- Adenosine Deaminase antibody LS-C745033 is an HRP-conjugated rabbit polyclonal antibody to bovine Adenosine Deaminase (ADA). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Adenosine deaminase | ADA1 | ADA | Adenosine aminohydrolase
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:5000
- UniProt Number
- P00813
- NCBI GenBank
- NM_000022 | NP_000013.2
- SwissProt
- P00813
Target
- Target
- Bovine ADA / Adenosine Deaminase
- Antigen Species
- Bovine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other sources is unknown.
- Gene Name
- ADA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Adenosine Deaminase [Calf Spleen] ADA
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl