Western blot of rabbit Anti-Adenosine Deaminase (Calf Spleen) antibody. Lane 1: Adenosine Deaminase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Adenosine Deaminase antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 44 kDa, 44 kDa for Adenosine Deaminase. Other band(s): None.
ADA / Adenosine Deaminase Antibody (Liquid)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Bovine |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744970-25
Catalog Number: LS-C744970
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- Description
- Adenosine Deaminase antibody LS-C744970 is an unconjugated rabbit polyclonal antibody to bovine Adenosine Deaminase (ADA). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Adenosine deaminase | ADA1 | ADA | Adenosine aminohydrolase
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:5000
- UniProt Number
- P00813
- NCBI GenBank
- NM_000022 | NP_000013.2
- SwissProt
- P00813
Target
- Target
- Bovine ADA / Adenosine Deaminase
- Antigen Species
- Bovine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species may occur but have not been specifically determined.
- Gene Name
- ADA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Adenosine Deaminase [Calf Spleen].
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl