Frozen section of human small intestine immunostained with SM1 (1:1000)
ACTA2 / Smooth Muscle Actin Antibody (clone IA4)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Other, Western Blot |
| Reactivity: | Chicken, Goat, Human, Mouse, Pig, Rat, Sheep |
| Format: | Liquid |
$480.00each
Catalog Number: LS-C146642-50
Catalog Number: LS-C146642
For bulk orders or custom formulations:
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- Description
- Smooth Muscle Actin antibody LS-C146642 is an unconjugated mouse monoclonal antibody to Smooth Muscle Actin (ACTA2) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, EM, IHC and WB.
- Application
- ELISA, IHC, IHC-F, Other, WB
- Reactivity
- Ck, Gt, Hu, Ms, Por, Rt, Sh
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ACTA2 | ACTVS | Alpha-actin-2 | Alpha-cardiac actin | AAT6 | Actin, aortic smooth muscle | ACTSA | Smooth Muscle Actin | MYMY5 | SMA
- Recommended Dilution: IHC-F
- 1:10 - 1:100
- Recommended Dilution: WB
- 1:10000 - 1:50000
- UniProt Number
- P62736
- NCBI GenBank
- NM_001613 | NP_001604.1
- SwissProt
- P62736
Target
- Target
- Human ACTA2 / Smooth Muscle Actin
- Antigen Species
- Human
- Epitope
- Reacts exclusively with alpha-smooth muscle actin, which is typical for vascular and visceral smooth muscle cells, but which is also present in myofibroblasts. The epitope is Ac-EEED, and is highly conserved. Therefore, the antibody cross-reacts with many species including protochordates, lower craniates and mammals.
- Gene Name
- ACTA2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- IA4
- Isotype
- IgG2a
Immunogen
- Immunogen
- Peptide comprising the first 10 amino acids of alpha-smooth muscle actin with an acetylated N-Terminus coupled to keyhole limpet hemocyanin via the C-Terminus cysteine (Ac-EEEDSTALVC).
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS