Immunofluorescence staining of HepG2 cells diluted at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ACTA1 / Skeletal Muscle Actin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Recombinant Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$323.00each
Catalog Number: LS-C675296-50
Catalog Number: LS-C675296
For bulk orders or custom formulations:
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- Description
- Skeletal Muscle Actin antibody LS-C675296 is an unconjugated rabbit recombinant monoclonal antibody to human Skeletal Muscle Actin (ACTA1). Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Recombinant Antibodies
- Alternative Names
- ACTA | Actin, alpha skeletal muscle | ACTA1 | Alpha-actin-1 | CFTD | CFTD1 | CFTDM | MPFD | NEM1 | NEM2 | ASMA | NEM3
- UniProt Number
- P68133
- NCBI GenBank
- NM_001100 | NP_001091.1
- SwissProt
- P68133
Target
- Target
- Human ACTA1 / Skeletal Muscle Actin
- Antigen Species
- Human
- Gene Name
- ACTA1
Antibody
- Host Species
- Rabbit
- Clonality
- Recombinant Monoclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthesized peptide
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- PBS|150 mM NaCl