LSBio
IHC-plus™ ACTA1 / Skeletal Muscle Actin Antibody (clone 3B3)

Anti-ACTA1 / ASMA antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ ACTA1 / Skeletal Muscle Actin Antibody (clone 3B3)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Goat, Human, Pig, Rabbit, Rat
Format:Liquid
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$470.00each
Catalog Number: LS-B7159-50

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Overview

Description
Skeletal Muscle Actin antibody LS-B7159 is an unconjugated mouse monoclonal antibody to Skeletal Muscle Actin (ACTA1) from human. It is reactive with human, rat, goat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-F, IHC-P, WB
Reactivity
Gt, Hu, Por, Rb, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
ACTA | Actin, alpha skeletal muscle | ACTA1 | Alpha-actin-1 | CFTD | CFTD1 | CFTDM | MPFD | NEM1 | NEM2 | ASMA | NEM3
Recommended Dilution: IHC-F
1:100 - 1:1000
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
P68133
SwissProt
P68133

Target

Target
ACTA1 / Skeletal Muscle Actin
Antigen Species
Human
Specificity
Highly specific for alpha- skeletal actin, and does not cross react with other actin isoforms. The epitope recognized by 3B3 is highly conserved. Therefore the antibody cross-reacts with many other species.
Epitope
Highly specific for alpha- skeletal actin, and does not cross react with other actin isoforms. The epitope recognized by 3B3 is highly conserved. Therefore the antibody cross-reacts with many other species.
Gene Name
ACTA1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
3B3
Origin Species
Human

Immunogen

Immunogen
Peptide comprising the N-Terminus nonapeptide of alpha-skeletal actin with an acetylated N-Terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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