Western blot analysis of extracts of various cell lines, using ASIC3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
ACCN3 / ASIC3 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C496975-20
Catalog Number: LS-C496975
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- ASIC3 antibody LS-C496975 is an unconjugated rabbit polyclonal antibody to ASIC3 (ACCN3) from human. It is reactive with mouse and rat. Validated for WB.
- Application
- IHC, WB
- Reactivity
- Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Acid-sensing ion channel 3 | Acid-sensing ion channel | ASIC3 | ACCN3 | HASIC3 | HTNaC1 | Modulatory subunit of ASIC2a | SLNAC1 | DRASIC | Testis sodium channel 1 | TNaC1
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q9UHC3
- NCBI GenBank
- NM_020321 | NP_064717.1
- SwissProt
- Q9UHC3
Target
- Target
- Human ACCN3 / ASIC3
- Antigen Species
- Human
- Gene Name
- ASIC3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 62-320 of human ASIC3 (NP_064717.1). ERVRYYREFHHQTALDERESHRLIFPAVTLCNINPLRRSRLTPNDLHWAGSALLGLDPAEHAAFLRALGRPPAPPGFMPSPTFDMAQLYARAGHSLDDMLLDCRFRGQPCGPENFTTIFTRMGKCYTFNSGADGAELLTTTRGGMGNGLDIMLDVQQEEYLPVWRDNEETPFEVGIRVQIHSQEEPPIIDQLGLGVSPGYQTFVSCQQQQLSFLPPPWGDCSSASLNPNYEPEPSDPLGSPSPSPSPPYTLMGCRLACE
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS