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IHC-plus™ SMO / Smoothened Antibody (N-Terminus)

Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)

IHC-plus™ SMO / Smoothened Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general)
Reactivity:Bovine, Dog, Hamster, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Pig, Rabbit, Rat
Format:Liquid
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$449.00each
Catalog Number: LS-A2666-50

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Overview

Description
Smoothened antibody LS-A2666 is an unconjugated rabbit polyclonal antibody to Smoothened (SMO) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Application
IHC
Reactivity
Bov, Can, Ham, Hr, Hu, Mml, Ms, NHP, Por, Rb, Rt
Intended Use
RUO
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Frizzled family member 11 | FZD11 | Gx | Protein Gx | SMOH | Smoothened | SMO | Smoothened homolog
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
Q99835
SwissProt
Q99835

Target

Target
SMO / Smoothened
Antigen Species
Hu
Specificity
N-terminus of SMO from human, mouse, rat and other mammals. BLAST analysis of the peptide immunogen showed no homology with other human proteins
Epitope
Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Gene Name
SMO

Antibody

Host Species
Rb
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic 15 amino acid peptide from N-Terminus extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.1% Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Vertebrate Smoothened functions at the primary cilium. Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY and Reiter JF. Nature. 2005;437:1018-21. PubMed: 16136078

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