LSBio
IHC-plus™ Polyclonal Rabbit anti-Mouse Leptin Antibody (IHC, WB)

Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Mouse Leptin Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse
Format:Liquid
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$490.00each
Catalog Number: LS-B1459-50

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Overview

Description
Leptin antibody LS-B1459 is an unconjugated rabbit polyclonal antibody to Leptin from mouse. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Leptin | LEP | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Recommended Dilution: ELISA
1:1000 - 1:5000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P41159
SwissProt
P41159

Target

Target
Leptin
Antigen Species
Mouse
Specificity
The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown.
Epitope
The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown.
Gene Name
LEP

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.01% Sodium Azide
Concentration
85 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Hypoplastic left heart syndrome is associated with structural and vascular placental abnormalities and leptin dysregulation. Jones HN, Olbrych SK, Smith KL, Cnota JF, Habli M, Ramos-Gonzales O, Owens KJ, Hinton AC, Polzin WJ, Muglia LJ, Hinton RB. Placenta. 2015;36:1078-86. PubMed: 26278057

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