LSBio
IHC-plus™ EZH2 Antibody (Internal)

Anti-EZH2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ EZH2 Antibody (Internal)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B2528-50

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Overview

Description
EZH2 antibody LS-B2528 is an unconjugated rabbit polyclonal antibody to human EZH2 (Internal). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ENX1 | Enhancer of zeste homolog 2 | EZH2 | KMT6 | KMT6A | Lysine N-methyltransferase 6 | WVS2 | Enhancer of zeste | ENX-1
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
0.5 - 2 µg/ml
UniProt Number
Q15910
SwissProt
Q15910

Target

Target
EZH2
Antigen Species
Hu
Specificity
Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse.
Epitope
Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse.
Gene Name
EZH2

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
Synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein.

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.25 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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