Western Blot of rabbit Anti-Histone H3 antibody. Lane 1: Hela WCL. Lane 2: Hela Nuclear Extract. Load: 10 µg per lane. Primary antibody: Histone H3 antibody at 1.0 µg/ml for 1 hr at RT. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~15 kDa, ~15 kDa for Histone H3.

Histone H3 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Chicken, Dog, Fruit Fly, Helminth / Nematode, Human, Mouse, Non-Human Primate, Plant, Rat, Xenopus
Format:	Liquid

$311.00each

Catalog Number: LS-C744996-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Histone H3 antibody LS-C744996 is an unconjugated rabbit polyclonal antibody to Histone H3 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Can, Ck, Dros, Helm, Hu, Ms, NHP, Plnt, Rt, Xen
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 5 ug/ml
Recommended Dilution: WB: 1 µg/ml

Target

Target: Human Histone H3
Antigen Species: Hu

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.
Immunogen Type: Pep

Format

Conjugate/Label: Unconj
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.98 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Prot A

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl