Anti-Rabbit IgG Secondary Antibody
Catalog Number: LS-C682348-500
500 µg / $301.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Bovine
Format: Liquid
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) antibody Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule Lane 2: Rabbit IgG F(ab) Fragment Lane 3: Rabbit IgG F(c) Fragment Lane 4: Rabbit IgM Whole Molecule Lane 5: Normal Rabbit Serum All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Goat Anti-Rabbit IgG Secondary Antibody (Atto 488)
Secondary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Immunofluorescence, Other, Western Blot |
| Reactivity: | Rabbit |
| Format: | Lyophilized |
$317.00each
Catalog Number: LS-C745593-100
Catalog Number: LS-C745593
For bulk orders or custom formulations:
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- Description
- Rabbit IgG antibody LS-C745593 is an Atto 488-conjugated goat polyclonal antibody. Validated for FLISA, IF and WB.
- Application
- IF, Other, WB
- Reactivity
- Rb
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: IF/ICC
- 1:5000
- Recommended Dilution: Other
- FLISA: 1:20000
- Recommended Dilution: WB
- 1:10000
Target
- Target
- Rabbit IgG
- Antigen Species
- Rabbit
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Rabbit IgG whole molecule
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Atto 488
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
