Anti-Rabbit IgG Secondary Antibody
Catalog Number: LS-C682348-500
500 µg / $301.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Bovine
Format: Liquid
Western blot of Peroxidase conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 25 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:40000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti-Rabbit IgG Secondary Antibody (HRP)
Secondary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot |
| Reactivity: | Rabbit |
| Format: | Lyophilized |
$274.00each
Catalog Number: LS-C153932-100
Catalog Number: LS-C153932
For bulk orders or custom formulations:
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- Description
- Rabbit IgG antibody LS-C153932 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
- Application
- DB, ELISA, IHC, Other, WB
- Reactivity
- Rb
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:10000 - 1:100000
- Recommended Dilution: IHC
- 1:500 - 1:5000
- Recommended Dilution: WB
- 1:5000 - 1:40000
Target
- Target
- Rabbit IgG
- Antigen Species
- Rabbit
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross-reactivity against human immunoglobulins.
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Rabbit IgG whole molecule
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
