Western Blot of rabbit anti-Beta Catenin antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: Beta catenin antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 85 kDa for Beta Catenin.
CTNNB1 / Beta Catenin Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745354-25
Catalog Number: LS-C745354
For bulk orders or custom formulations:
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- Description
- Beta Catenin antibody LS-C745354 is an unconjugated rabbit polyclonal antibody to Beta Catenin (CTNNB1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Mouse, Rat, Xenopus, Zebrafish
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Beta-catenin | Catenin beta-1 | CTNNB | CTNNB1 | Beta Catenin
- Recommended Dilution: ELISA
- 1:25000
- Recommended Dilution: IHC
- 5 - 10 µg/ml
- Recommended Dilution: WB
- 1:500
- UniProt Number
- P35222
- NCBI GenBank
- NM_001904 | NP_001895.1
- SwissProt
- P35222
Target
- Target
- Human CTNNB1 / Beta Catenin
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- CTNNB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-Terminus.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.15 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl