Immunofluorescence staining of CGRRF1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CGRRF1 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

CGR19 / CGRRF1 Antibody (Preservative Free)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin
Reactivity:	Human
Format:	Liquid

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$309.00each

Catalog Number: LS-C814767-100

Unit Size

Quantity

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Overview

Description: CGRRF1 antibody LS-C814767 is an unconjugated rabbit polyclonal antibody to human CGRRF1 (CGR19). Validated for ICC, IF and IHC.
Application: ICC, IF, IHC-P
Reactivity: Hu
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CGR19 | CGRRF1 | RING finger protein 197 | Cell growth regulator CGR19 | GCRRF1 | RNF197
Recommended Dilution: IF/ICC: 1:300 - 1:10000
Recommended Dilution: IHC-P: 1:200 - 1:1000
UniProt Number: Q99675
NCBI GenBank: NM_006568, NP_006559.1
SwissProt: Q99675

Target

Target: Human CGR19 / CGRRF1
Antigen Species: Hu
Epitope: Human CGR19/CGRRF1
Gene Name: CGRRF1

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG
Origin Species: Hu

Immunogen

Immunogen: A synthetic peptide corresponding to the center region of the Human CGR19/CGRRF1
Immunogen Type: SP-Lin

Format

Conjugate/Label: Unconj
Format: Liquid
Formulation: 0.2 µm filtered solution in PBS
Volume: 100 Microliter
Preservative: No
Purification Method: Prot A

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS