Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CGR19 / CGRRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115220-100
Catalog Number: LS-G115220
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CGR19(CGRRF1) (NM_006568), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CGR19 | CGRRF1 | RING finger protein 197 | Cell growth regulator CGR19 | GCRRF1 | RNF197
- Origin Species
- Human
- NCBI GenBank
- NM_006568
- SwissProt
- Q99675
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAVFLVTLY EYSPLFYIAV VFTCFIVTTG LVLGWFGWDV PVILRNSEET QFSTRVFKKQ61MRQVKNPFGL EITNPSSASI TTGITLTTDC LEDSLLTCYW GCNVQKLYEA LQKHVYCFRI121STPQALEDAL YSEYLYQEQY FIKKDSKEEI YCQLPRDTKI EDFGTVPRSR YPLVALLTLA181DEDDREIYDI ISMVSVIHIP DRTYKLSCRI LYQYLLLAQG QFHDLKQLFM SANNNFTPSN241NSSSEEKNTD RSLLEKVGLS ESEVEPSEEN SKDCVVCQNG TVNWVLLPCR HTCLCDGCVK301YFQQCPMCRQ FVQESFALCS QKEQDKDKPK TLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
332 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.