Western Blot of rabbit anti-p27kip1pS140 antibody. Lane 1: 293 cells untransfected. Lane 2: 293 cells untransfected and 5Gy irradiated. Lane 3: 293 cells transfected with HA-p27wt. Lane 4: 293 cells transfected with HA-p27wt and 5Gy irradiated. Lane 5: 293 cells transfected with HA-p27S140A. Lane 6: 293 cells transfected with HA-p27S140A and 5Gy irradiated. Load: 20 µg per lane. Primary antibody: p27kip1pS140 antibody at 1:250 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TTBS overnight at 4°C. Predicted/Observed size: 27 kDa for p27kip1.
CDKN1B / p27 Kip1 Antibody (phospho-Ser140)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745494-25
Catalog Number: LS-C745494
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- Description
- P27 Kip1 antibody LS-C745494 is an unconjugated rabbit polyclonal antibody to human p27 Kip1 (CDKN1B) (pSer140). Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDKN1B | KIP1 | MEN1B | P27 Kip1 | p27KIP1 | MEN4 | CDKN4
- Recommended Dilution: ELISA
- 1:10000 - 1:40000
- Recommended Dilution: WB
- 1:500 - 1:4000
- UniProt Number
- P46527
- NCBI GenBank
- NM_004064, NP_004055.1
- SwissProt
- P46527
Target
- Target
- Human CDKN1B / p27 Kip1
- Antigen Species
- Hu
- Gene Name
- CDKN1B
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.68 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl