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Antibodies > CDK5 > LS-C392596
 
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Catalog Number Size Price
LS-C392596-200 200 µl $393 
CDK5 Antibody - Antibody staining CDK5 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
CDK5 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
CDK5 Antibody - All lanes: Anti-CDK5 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: HeLa whole cell lysate. Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
CDK5 Antibody - Overlay histogram showing K562 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
CDK5 Antibody - Antibody staining CDK5 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
CDK5 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
CDK5 Antibody - All lanes: Anti-CDK5 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: HeLa whole cell lysate. Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
CDK5 Antibody - Overlay histogram showing K562 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Monoclonal Mouse anti‑Human CDK5 Antibody (clone 1552CT262.105.8, IHC, IF, WB) LS‑C392596

Monoclonal Mouse anti‑Human CDK5 Antibody (clone 1552CT262.105.8, IHC, IF, WB) LS‑C392596

Submit Review LSBio Guarantee
Antibody:
CDK5 Mouse anti-Human Monoclonal (1552CT262.105.8) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392596-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CDK5 Mouse anti-Human Monoclonal (1552CT262.105.8) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CDK5 antibody LS-C392596 is an unconjugated mouse monoclonal antibody to CDK5 from human. It is reactive with human and mouse. Validated for Flow, IF, IHC, Peptide-ELISA and WB.
Target
Human CDK5
Synonyms
CDK5 | CDKN5 | Cell division protein kinase 5 | Crk6 | Cyclin-dependent kinase 5 | Protein kinase CDK5 splicing | PSSALRE | TPKII catalytic subunit
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1552CT262.105.8
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDK5
Q00535 NM_004935 NP_004926.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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