Human CD55

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

BRIC216

Unconjugated

Protein G purified

Unmodified

Human fibroblast cell line

Recognizes the CD55 antigen, a 70kD glycoprotein also known as Decay Accelerating Factor (DAF). Distributed on erythrocytes and other circulating blood cells and also on cells in non-hemopoietic tissue particularly epithelium and endothelium. Found specifically at the fetal-maternal interfaces in placenta. CD55 has reduced expression on individuals with paroxysmal nocturnal hemoglobinuria. Has a functional binding affinity to erythrocytes of 8.7 x 107 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Recognizes the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF.

• Western blot
• Immunoprecipitation
• Flow Cytometry

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul Method sheets are available on request.

TBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08174 NCBI: NM_000574 NP_000565.1