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Anti-DDX / AKR1C3 Antibody (aa311-323) IHC-plus™ LS-B2295

Note: This antibody replaces LS-C54477, LS-C68877
Catalog Size Price
LS-B2295-50 50 µg (0.5 mg/ml) Unavailable

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Immunohistochemistry Image
Anti-DDX / AKR1C3 Antibody (aa107-135, Biotin) LS-C251111
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Anti-DDX / AKR1C3 Antibody IHC-plus™ LS-B14868
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Anti-DDX / AKR1C3 Antibody LS-C659520
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100% Guaranteed Publications (1)
Goat Polyclonal to Human DDX / AKR1C3
IHC - Paraffin, Western blot, ELISA


Human DDX / AKR1C3
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:32000) & (0.01 - 0.1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

DDX / AKR1C3 antibody was raised against synthetic peptide C-FASHPNYPYSDEY from the C-terminus of human AKR1C3 (NP_003730.4). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Orangutan (92%), Gibbon (92%), Monkey (92%).
LS-E25822 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2295. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human AKR1C3.
Immunohistochemistry: LS-B2295 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2295 was determined to be 5 ug/ml.


Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

Publications (1)

Ovarian Brenner tumour: a morphologic and immunohistochemical analysis suggesting an origin from fallopian tube epithelium. Kuhn E, Ayhan A, Shih IeM, Seidman JD, Kurman RJ. European journal of cancer (Oxford, England : 1990). 2013 Dec;49:3839-49. [Full Text Article] [PubMed:24012099]

About DDX / AKR1C3

P42330 AF110411 IPI00031381.1

AKR1C3 Antibody, 3-alpha-HSD type 2 Antibody, 17-beta-HSD 5 Antibody, 3-alpha-HSD type II, brain Antibody, DD3 Antibody, DDH1 Antibody, DDX Antibody, Dihydrodiol dehydrogenase 3 Antibody, Dihydrodiol dehydrogenase X Antibody, DD-3 Antibody, HA1753 Antibody, HAKRe Antibody, HAKRB Antibody, HSD17B5 Antibody, KIAA0119 Antibody, Kia0119 Antibody, Prostaglandin F synthase Antibody, PGFS Antibody, Truncated aldoketo reductase a Antibody, HluPGFS Antibody, Indanol dehydrogenase Antibody

DDX / AKR1C3 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2.


Anti-AKR1C3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2295 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.03 ug/ml) staining of human breast lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 2/19/2018

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