Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DDX / AKR1C3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117121-100
Catalog Number: LS-G117121
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AKR1C3 (NM_003739), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 3-alpha-HSD type 2 | 17-beta-HSD 5 | 3-alpha-HSD type II | brain | DD3 | DDH1 | DDX | Dihydrodiol dehydrogenase 3 | Dihydrodiol dehydrogenase X | DD-3 | AKR1C3 | HA1753 | HAKRe | HAKRB | HSD17B5 | KIAA0119 | Kia0119 | Prostaglandin F synthase | PGFS | Truncated aldoketo reductase a | HluPGFS | Indanol dehydrogenase
- Origin Species
- Human
- NCBI GenBank
- AF110411
- SwissProt
- P42330
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ61VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM121SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP181GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV241LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD301RNLHYFNSDS FASHPNYPYS DEYTRTRPLE QKLISEEDLA ANDILDYKDD DDKV
354 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.