IHC‑plus™ Monoclonal Mouse anti‑Human Cytokeratin 8+18+19 Antibody (clone 2A4, Concentrated, IHC, WB) LS‑B5567
IHC‑plus™ Monoclonal Mouse anti‑Human Cytokeratin 8+18+19 Antibody (clone 2A4, Concentrated, IHC, WB) LS‑B5567
Note: This antibody replaces LS-C134860
Antibody:
Cytokeratin 8+18+19 Mouse anti-Human Monoclonal (Concentrated) (2A4) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Format:
Unconjugated, Concentrated
Overview
Antibody:
Cytokeratin 8+18+19 Mouse anti-Human Monoclonal (Concentrated) (2A4) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Format:
Unconjugated, Concentrated
Specifications
Description
Cytokeratin 8+18+19 antibody LS-B5567 is an unconjugated mouse monoclonal antibody to human Cytokeratin 8+18+19. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Cytokeratin 8+18+19
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Fast Protein Liquid Chromatography (FPLC)
Modifications
Concentrated
Immunogen
Cytokeratins purified from pig kidney eptihelial cells
Specificity
The antibody recognizes cytokeratin nos. 8, 18 and 19 typical for simple epithelial cells. Monoclonal antibody to cytokeratin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Cytokeratins isolated from cultured pig kidney epithelial cells were used as immunogen.
Applications
- IHC
- IHC - Paraffin (1:100)
- IHC - Frozen (1:200)
- Western blot (1:1000 - 1:4000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemical stainings (frozen sections) and western blotting. Working dilution in western blotting is at least 1:1000. This antibody has been tested with human samples, although raised against porcine, it has not been tested with porcine samples.
Presentation
PBS, 0.1% Sodium Azide, 1% BSA
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-Keratin 8/18/19 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Keratin 8/18/19 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Source:
Human
Tag:
Fc, N-terminus
Reactivity:
Human
Range:
3.12-200 ng/ml
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Flow Cytometry
Reactivity:
Human
Range:
0.156-10 ng/ml
Reactivity:
Human
Range:
0.31-20 ng/ml
Species:
Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/18/2024