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Anti-CLDN5 / Claudin 5 Antibody IHC-plus™ LS-B2331

Note: This antibody replaces LS-C6158
Catalog Size Price
LS-B2331-50 50 µg (0.5 mg/ml) Unavailable

Most Popular CLDN5 / Claudin 5 Antibodies

Anti-CLDN5 / Claudin 5 Antibody IHC-plus™ LS-B2331
Mouse Monoclonal (IgG1) to Mouse CLDN5 / Claudin 5
Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CLDN5 / Claudin 5 Antibody (C-Terminus) IHC-plus™ LS-B3064
Rabbit Polyclonal to Mouse CLDN5 / Claudin 5
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CLDN5 / Claudin 5 Antibody (aa169-218) IHC-plus™ LS-B5971
Rabbit Polyclonal (IgG) to Human CLDN5 / Claudin 5
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CLDN5 / Claudin 5 Antibody (aa169-218) LS-C291250
Rabbit Polyclonal (IgG) to Human CLDN5 / Claudin 5
Human, Mouse, Rat
IHC, ELISA
Unconjugated
Anti-CLDN5 / Claudin 5 Antibody (C-Terminus) LS-C352946
Rabbit Polyclonal to Human CLDN5 / Claudin 5
Human, Mouse, Rat, Bovine, Pig, Rabbit
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Mouse Monoclonal (IgG1) to Mouse CLDN5 / Claudin 5
Mouse, Rat
IHC - Paraffin, Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Mouse CLDN5 / Claudin 5
Mouse
Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Synthetic peptide derived from the mouse Claudin-5 protein.
Recognizes rat endogenous Claudin-5 protein at ~22-24kD. Species cross-reactivity: WB: mouse, IHC: human.
Immunohistochemistry: LS-B2331 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2331 was determined to be 5-10 ug/ml. Positive control: Rat lung, kidney, and small intestine homogenates and mouse lung homogenates.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CLDN5 / Claudin 5

O00501 NM_003277 NP_003268.2

CLDN5 Antibody, BEC1 Antibody, AWAL Antibody, Claudin-5 Antibody, CPETRL1 Antibody, TMDVCF Antibody, TMVCF Antibody, Claudin 5 Antibody

CLDN5 / Claudin 5 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunohistochemistry

Immunohistochemistry
Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.

Requested From: 
Date Requested: 10/18/2017

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