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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CLDN5 / Claudin 5 Antibody IHC‑plus™ LS‑B2331 Best Seller
Note: This antibody replaces LS-C6158
Claudin 5 antibody LS-B2331 is an unconjugated mouse monoclonal antibody to Claudin 5 (CLDN5) from mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2331-50
50 µg (0.5 mg/ml)
Unavailable

Popular CLDN5 / Claudin 5 Products

Anti-CLDN5 / Claudin 5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3064 concentration 15 ug/ml.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-CLDN5 / Claudin 5 antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5971 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of Claudin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Pig, Rabbit
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of Claudin-5 antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

Claudin 5 antibody LS-B2331 is an unconjugated mouse monoclonal antibody to Claudin 5 (CLDN5) from mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CLDN5 / Claudin 5
CLDN5 / Claudin 5 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. O00501 NM_003277 NP_003268.2

CLDN5 Antibody, BEC1 Antibody, AWAL Antibody, Claudin-5 Antibody, CPETRL1 Antibody, TMDVCF Antibody, TMVCF Antibody, Claudin 5 Antibody


Specifications

Target
Mouse CLDN5 / Claudin 5
Host
Mouse
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from the mouse Claudin-5 protein.
Specificity
Recognizes rat endogenous Claudin-5 protein at ~22-24kD. Species cross-reactivity: WB: mouse, IHC: human.
Usage
Immunohistochemistry: LS-B2331 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2331 was determined to be 5-10 ug/ml. Positive control: Rat lung, kidney, and small intestine homogenates and mouse lung homogenates.
Presentation
PBS, pH 7.4, 0.09% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.
Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.

Immunohistochemistry

Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.
Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.

Immunohistochemistry

Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.
Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.

Immunohistochemistry

Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.
Anti-CLDN5 / Claudin 5 antibody IHC of rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2331 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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