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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCNT1 / Cyclin T1 Antibody (Internal) IHC‑plus™ LS‑B351 Best Seller
Note: This antibody replaces LS-C18945
Cyclin T1 antibody LS-B351 is an unconjugated rabbit polyclonal antibody to Cyclin T1 (CCNT1) from human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B351-50
50 µl (75 mg/ml)
Unavailable

Popular CCNT1 / Cyclin T1 Products

CCNT1 Antibody western blot of K562 cell line lysates (35 ug/lane). The CCNT1 antibody detected the CCNT1 protein (arrow).
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Anti-CCNT1 / Cyclin T1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10078 concentration 10 ug/ml.
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Antibody
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Detection of Human Cyclin T1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin T1 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

Cyclin T1 antibody LS-B351 is an unconjugated rabbit polyclonal antibody to Cyclin T1 (CCNT1) from human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About CCNT1 / Cyclin T1
CCNT1 / Cyclin T1 is a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. O60563 NM_001240 NP_001231.2

CCNT1 Antibody, CCNT Antibody, CDK9-associated C-type protein Antibody, Cyclin T1b Antibody, CYCT1 Antibody, Cyclin T1 Antibody, HIVE1 Antibody, Cyclin C-related protein Antibody, Cyclin-T Antibody, Cyclin-T1 Antibody


Specifications

Target
Human CCNT1 / Cyclin T1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Sterile filtered
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
Internal
Specificity
Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
Immunohistochemistry: LS-B351 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B351 was determined to be 1:500.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.
Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.

Immunohistochemistry

Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.
Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.

Immunohistochemistry

Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.
Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.

Immunohistochemistry

Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.
Anti-CCNT1 / Cyclin T1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B351 dilution 1:500.


Requested From: 
Date Requested: 6/24/2018

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