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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CAND2 / TIP120B Antibody (C‑Terminus) IHC‑plus™ LS‑B348 Best Seller
Note: This antibody replaces LS-C19123
TIP120B antibody LS-B348 is an unconjugated rabbit polyclonal antibody to human TIP120B (CAND2). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Catalog
Size
Price
LS-B348-50
50 µl (85 mg/ml)
Unavailable

Popular CAND2 / TIP120B Products

Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
Detection of Human TIP120B by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TIP120B antibody used for IP at 6 ug/mg lysate. TIP120B was also immunoprecipitated by rabbit anti-TIP120B antibody BL14617. For blotting immunoprecipitated TIP120B, was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Species: Human, Mouse, Bovine, Horse, Pig
Applications: Western blot, Immunoprecipitation
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot

Product Description

TIP120B antibody LS-B348 is an unconjugated rabbit polyclonal antibody to human TIP120B (CAND2). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
About CAND2 / TIP120B
Probable assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. O75155 NP_036430.1

CAND2 Antibody, KIAA0667 Antibody, p120 CAND2 Antibody, TBP interacting protein Antibody, TIP120B Antibody, TBP-interacting protein 120B Antibody, Tp120b Antibody


Specifications

Target
Human CAND2 / TIP120B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CAND2 / TIP120B antibody was raised against synthetic peptide from human CAND2 / TIP120B.
Epitope
C-Terminus
Specificity
Amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.
Usage
Immunohistochemistry: LS-B348 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B348 was determined to be 1:500.
Presentation
0.01% sodium azide.
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (3)

1
NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Liu J, Furukawa M, Matsumoto T, Xiong Y. Molecular cell. 2002 10:1511-8. [PubMed:12504025]
2
AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Cheng Y, Dai X, Zhao Y. Plant physiology. 2004 135:1020-6. [PubMed:15181201] [PMC:PMC514136]
3
Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. Feng S, Shen Y, Sullivan JA, Rubio V, Xiong Y, Sun TP, Deng XW. The Plant cell. 2004 16:1870-82. [PubMed:15208391] [PMC:PMC514167]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.
Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.

Immunohistochemistry

Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.
Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.

Immunohistochemistry

Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.
Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.

Immunohistochemistry

Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.
Anti-CAND2 / TIP120B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B348 dilution 1:500.


Requested From: 
Date Requested: 6/24/2018

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