Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAND2 / TIP120B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128433-100
Catalog Number: LS-G128433
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TIP120B(CAND2) (NM_001162499), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0667 | p120 CAND2 | TBP interacting protein | TIP120B | TBP-interacting protein 120B | Tp120b | CAND2
- Origin Species
- Human
- SwissProt
- O75155
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 135.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTAAFHISS LLEKMTSSDK DFRFMATSDL MSELQKDSIQ LDEDSERKVV KMLLRLLEDK61NGEVQNLAVK CLGPLVVKVK EYQVETIVDT LCTNMRSDKE QLRDIAGIGL KTVLSELPPA121ATGSGLATNV CRKITGQLTS AIAQQEDVAV QLEALDILSD MLSRLGVPLG AFHASLLHCL181LPQLSSPRLA VRKRAVGALG HLAAACSTDL FVELADHLLD RLPGPRVPTS PTAIRTLIQC241LGSVGRQAGH RLGAHLDRLV PLVEDFCNLD DDELRESCLQ AFEAFLRKCP KEMGPHVPNV301TSLCLQYIKH DPNYNYDSDE DEEQMETEDS EFSEQESEDE YSDDDDMSWK VRRAAAKCIA361ALISSRPDLL PDFHCTLAPV LIRRFKEREE NVKADVFTAY IVLLRQTQPP KGWLEAMEEP421TQTGSNLHML RGQVPLVVKA LQRQLKDRSV RARQGCFSLL TELAGVLPGS LAEHMPVLVS481GIIFSLADRS SSSTIRMDAL AFLQGLLGTE PAEAFHPHLP ILLPPVMACV ADSFYKIAAE541ALVVLQELVR ALWPLHRPRM LDPEPYVGEM SAVTLARLRA TDLDQEVKER AISCMGHLVG601HLGDRLGDDL EPTLLLLLDR LRNEITRLPA IKALTLVAVS PLQLDLQPIL AEALHILASF661LRKNQRALRL ATLAALDALA QSQGLSLPPS AVQAVLAELP ALVNESDMHV AQLAVDFLAT721VTQAQPASLV EVSGPVLSEL LRLLRSPLLP AGVLAAAEGF LQALVGTRPP CVDYAKLISL781LTAPVYEQAV DGGPGLHKQV FHSLARCVAA LSAACPQEAA STASRLVCDA RSPHSSTGVK841VLAFLSLAEV GQVAGPGHQR ELKAVLLEAL GSPSEDVRAA ASYALGRVGA GSLPDFLPFL901LEQIEAEPRR QYLLLHSLRE ALGAAQPDSL KPYAEDIWAL LFQRCEGAEE GTRGVVAECI961GKLVLVNPSF LLPRLRKQLA AGRPHTRSTV ITAVKFLISD QPHPIDPLLK SFIGEFMESL1021QDPDLNVRRA TLAFFNSAVH NKPSLVRDLL DDILPLLYQE TKIRRDLIRE VEMGPFKHTV1081DDGLDVRKAA FECMYSLLES CLGQLDICEF LNHVEDGLKD HYDIRMLTFI MVARLATLCP1141APVLQRVDRL IEPLRATCTA KVKAGSVKQE FEKQDELKRS AMRAVAALLT IPEVGKSPIM1201ADFSSQIRSN PELAALFESI QKDSASAPST DSMELSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1236 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.