Human C4BP / C4b-Binding Protein

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

10-07

Unconjugated

Protein A purified from ascites

Unmodified

Native human C4 binding protein

Recognizes complement component 4 binding protein (C4bp). There are three variants of C4bp, composed of alpha and beta chains. The predominant form is a 570 kD complex of 7 alpha chains and 1 beta chain. The other complexes formed are a 530 kD homoheptamer of alpha chains or a 500 kD complex of 6 alpha chains and 1 beta chain. C4bp regulates the activation of the classical complement pathway. It is a cofactor to the C3b/C4b inactivator, crucial to the proteolytic cleavage of C4b and important in cleavage of C3b. C4bp also regulates the C4bC2a complex (C3 convertase), accelerating its degradation by dissociating the complement fragment C2a. Both the C4bp alpha and beta chains interact with anticoagulant protein S and C4bp alpha binds protein S. C4bp is expressed in the blood serum.

• IHC
• IHC - Paraffin (5 µg/ml)
• ELISA

Immunohistochemistry: LS-B2251 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2251 was determined to be 5 ug/ml.

BBS, 0.1% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.