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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AVEN / PDCD12 Antibody (C‑Terminus) IHC‑plus™ LS‑B444 Best Seller
Note: This antibody replaces LS-C19455, LS-C122707
PDCD12 antibody LS-B444 is an unconjugated rabbit polyclonal antibody to PDCD12 (AVEN) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B444-50
50 µg (0.5 mg/ml)
Unavailable

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Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

PDCD12 antibody LS-B444 is an unconjugated rabbit polyclonal antibody to PDCD12 (AVEN) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About AVEN / PDCD12
Protects against apoptosis mediated by Apaf-1. Q9NQS1 NM_020371 NP_065104.1

AVEN Antibody, Cell death regulator Aven Antibody, Programmed cell death 12 Antibody, PDCD12 Antibody


Specifications

Target
Human AVEN / PDCD12
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E29960 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B444. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
Immunohistochemistry: LS-B444 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B444 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.
Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.

Western blot

Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.

Western blot

Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.

Immunohistochemistry

Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.
Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.

Western blot

Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.

Western blot

Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.

Immunohistochemistry

Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.
Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.

Western blot

Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.

Western blot

Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.

Immunohistochemistry

Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.
Anti-AVEN antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B444 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 ug/ml.

Western blot

Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 µg/mL in (A) the presence and (B) the absence of blocking peptide.

Western blot

Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
Western blot of Aven in Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.


Requested From: 
Date Requested: 6/20/2018

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