Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AVEN / PDCD12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111195-100
Catalog Number: LS-G111195
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AVEN (NM_020371), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AVEN | Cell death regulator Aven | Programmed cell death 12 | PDCD12
- Origin Species
- Human
- NCBI GenBank
- NM_020371
- SwissProt
- Q9NQS1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQAERGARGG RGRRPGRGRP GGDRHSERPG AAAAVARGGG GGGGGDGGGR RGRGRGRGFR61GARGGRGGGG APRGSRREPG GWGAGASAPV EDDSDAETYG EENDEQGNYS KRKIVSNWDR121YQDIEKEVNN ESGESQRGTD FSVLLSSAGD SFSQFRFAEE KEWDSEASCP KQNSAFYVDS181ELLVRALQEL PLCLRLNVAA ELVQGTVPLE VPQVKPKRTD DGKGLGMQLK GPLGPGGRGP241IFELKSVAAG CPVLLGKDNP SPGPSRDSQK PTSPLQSAGD HLEEELDLLL NLDAPIKEGD301NILPDQTSQD LKSKEDGEVV QEEEVCAKPS VTEEKNMEPE QPSTSKNVTE EELEDWLDSM361ISTRTRPLEQKLISEEDLAANDILDYKDDDDKV
362 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.