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Anti-Apg7 / ATG7 Antibody (aa558-569) IHC-plus™ LS-B330

Note: This antibody replaces LS-C18991, LS-C69114, LS-C56872

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Apg7 / ATG7 Antibodies

Anti-Apg7 / ATG7 Antibody (aa558-569) IHC-plus™ LS-B330
Rabbit Polyclonal to Human Apg7 / ATG7
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Apg7 / ATG7 Antibody (N-Terminus) IHC-plus™ LS-B2195
Rabbit Polyclonal to Human Apg7 / ATG7
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human Apg7 / ATG7
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human Apg7 / ATG7
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:15000 - 1:75000)

Specificity and Use

Apg7 / ATG7 antibody was raised against synthetic peptide from human ATG7.
aa558-569
aa 558-569 of Human Apg7 protein.
Immunohistochemistry: LS-B330 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B330 was determined to be 10 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

1
Autophagy mediates HIF2 alpha degradation and suppresses renal tumorigenesis. Yao J, Tripathi DN, Ding Z, Xu Y, Sun M, Zhang J, Bai S, German P, Hoang A, Zhou L, Jonasch D, Zhang X, Conti CJ, Efstathiou E, Tannir NM, Eissa NT, Mills GB, Walker CL, Jonasch E. Oncogene. Jul; [Full Text Article] [PubMed:24998849] [PMC:PMC4286517]

About Apg7 / ATG7

O95352 NM_006395 NP_006386.1

ATG7 Antibody, Apg7 Antibody, APG7L Antibody, APG7-LIKE Antibody, Autophagy related 7 Antibody, Autophagy-related protein 7 Antibody, HAGP7 Antibody, GSA7 Antibody

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition.

Immunohistochemistry

Immunohistochemistry
Anti-ATG7 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B330 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/7/2016

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