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Anti-ACS5 / ACSL5 Antibody (aa727-739) IHC-plus™ LS-B2761

Note: This antibody replaces LS-C54441, LS-C63414

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACS5 / ACSL5 Antibodies

Anti-ACS5 / ACSL5 Antibody (aa727-739) IHC-plus™ LS-B2761
Goat Polyclonal to Human ACS5 / ACSL5
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ACS5 / ACSL5 Antibody (aa470-519) IHC-plus™ LS-B5798
Rabbit Polyclonal (IgG) to Human ACS5 / ACSL5
Human, Monkey, Mouse, Rat, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ACS5 / ACSL5 Antibody LS-C335145
Rabbit Polyclonal (IgG) to Human ACS5 / ACSL5
Human, Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human ACS5 / ACSL5
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ACS5 / ACSL5
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (1:16000)

Specificity and Use

ACS5 / ACSL5 antibody was raised against synthetic peptide C-RTQIDSLYEHIQD from the C-terminus of human ACSL5 (NP_057318.2; NP_976313.1; NP_976314.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Panda (85%).
LS-E26096 - Lyophilized - 100 µg - $145.00
aa727-739
Human ACSL5. This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).
Immunohistochemistry: LS-B2761 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2761 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ACS5 / ACSL5

Q9ULC5 NM_016234 NP_057318.2

ACSL5 Antibody, ACS2 Antibody, ACS5 Antibody, FACL5 Antibody, Fatty acid coenzyme A ligase 5 Antibody, Phosphatidylinositol 4-kinase Antibody, LACS 5 Antibody

Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity).

Immunohistochemistry

Immunohistochemistry
Anti-ACSL5 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2761 concentration 5 ug/ml.

Western blot

Western blot
Antibody (1 ug/ml) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/3/2016

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