Western Blot of rabbit anti-Alpha Amylase Antibody Biotin Conjugated. Lane 1: Alpha Amylase solution. Load: 1/200 dilution of Alpha Amylase solution. Primary antibody: Alpha Amylase antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Alpha Amylase. Other band(s): proteins within Alpha Amylase solution.

Alpha Amylase Antibody (Liquid, Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:	Bacteria
Format:	Liquid

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$311.00each

Catalog Number: LS-C745128-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Alpha Amylase antibody LS-C745128 is a biotin-conjugated rabbit polyclonal antibody to bacillus amyloliquefaciens Alpha Amylase. Validated for ELISA, IP and WB.
Application: ELISA, IP, WB
Reactivity: Bac
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:5000 - 1:20000
Recommended Dilution: IP: 1:100
Recommended Dilution: WB: 1:500 - 1:5000

Target

Target: Bacillus amyloliquefaciens Alpha Amylase
Antigen Species: Bac
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens]. Cross reactivity against a-Amylase from other sources may occur but has not been specifically determined.

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: A-Amylase [Bacillus amyloliquefaciens].

Format

Conjugate/Label: Biotin
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: IEX

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl