Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
AKT2 Antibody (Internal, clone 11F6.B2)
Primary AntibodyLSBio Guarantee
| Antibody: | Rat Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745153-25
Catalog Number: LS-C745153
For bulk orders or custom formulations:
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- Description
- AKT2 antibody LS-C745153 is an unconjugated rat monoclonal antibody to human AKT2 (Internal). Validated for ELISA, Flow, IHC and WB.
- Application
- ELISA, FC, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC
- 20 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P31751
- NCBI GenBank
- NM_001626 | NP_001617.1
- SwissProt
- P31751
Target
- Target
- Human AKT2
- Antigen Species
- Human
- Epitope
- The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
- Gene Name
- AKT2
Antibody
- Host Species
- Rat
- Clonality
- Monoclonal
- Clone
- 11F6.B2
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
