Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4°C.

AKT1 Antibody (Internal, clone 5E5.F5.D7, FITC)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Lyophilized

$594.00each

Catalog Number: LS-C744918-50

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Overview

Description: AKT1 antibody LS-C744918 is an FITC-conjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Application: ELISA, FC, IF, IHC, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
UniProt Number: P31749
NCBI GenBank: NM_005163 | NP_005154.2
SwissProt: P31749

Target

Target: Human AKT1
Antigen Species: Human
Epitope: The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Gene Name: AKT1

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 5E5.F5.D7
Isotype: IgG2a
Light Chain: Kappa Light Chain
Origin Species: Human

Immunogen

Immunogen: Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Fluorescein Isothiocyanate
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.5 M NaCl