Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Immunofluorescence Microscopy of Mouse Anti-AKTpS473 antibody using STED nanoscopy to evaluate AKT activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved,unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Staining: Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKTpS473 at the cell periphery.
AKT1 Antibody (phospho-Ser473, clone 17F6.B11)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate |
| Format: | Liquid |
$2652.00each
Catalog Number: LS-C744831-1
Catalog Number: LS-C744831
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- AKT1 antibody LS-C744831 is an unconjugated mouse monoclonal antibody to AKT1 (pSer473) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC, WB
- Reactivity
- Hu, Ms, NHP
- Predicted Reactivity
- Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: IF/ICC
- 1:500 - 1:3000
- Recommended Dilution: IHC
- 20 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- P31749
- NCBI GenBank
- NM_005163 | NP_005154.2
- SwissProt
- P31749
Target
- Target
- Human AKT1
- Antigen Species
- Human
- Epitope
- This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
- Gene Name
- AKT1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 17F6.B11
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
