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PRF1 / Perforin

perforin 1 (pore forming protein)

Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.

Gene Name: perforin 1 (pore forming protein)
Synonyms: PRF1, HPLH2, Perforin, p1, Perforin1, PFP, Cytolysin, FLH2, Perforin-1
Target Sequences: NM_005041 NP_005032.2 P14222

Publications (2)

1
Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity. Wood SM, Meeths M, Chiang SC, Bechensteen AG, Boelens JJ, Heilmann C, Horiuchi H, Rosthj S, Rutynowska O, Winiarski J, Stow JL, Nordenskjld M, Henter JI, Ljunggren HG, Bryceson YT. Blood. 2009 114:4117-27. [PubMed:19704116]
2
Anti-PD-L1 prolongs survival and triggers T cell but not humoral anti-tumor immune responses in a human MUC1-expressing preclinical ovarian cancer model. Mony JT, Zhang L, Ma T, Grabosch S, Tirodkar TS, Brozick J, Tseng G, Elishaev E, Edwards RP, Huang X, Vlad AM. Cancer immunology, immunotherapy : CII. 2015 September;64:1095-108. [Full Text Article] [PubMed:25998800] [PMC:PMC4545381] Related Antibodies: LS-C18632.

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Products
Antibodies (68)
Primary (68)
PRF1 / Perforin (68)
Human (48)
Mouse (14)
Rat (14)
Bovine (5)
Gibbon (3)
Horse (3)
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IHC (13)
IHC-Fr (10)
IHC-P (11)
WB (48)
Flo (9)
ELISA (13)
ICC (3)
IF (2)
IP (7)
Peptide-ELISA (1)
rabbit (48)
mouse (19)
rat (1)
Product Group
IHCPlus (1)
IgG (21)
IgG1 (4)
IgG2a (2)
IgG2a,k (1)
IgG2b (4)
IgG2b,k (7)
IgG2c,k (2)
monoclonal mc (20)
polyclonal pc (48)
3B4 (1)
5B10 (2)
BOR21 (1)
CB5.4 (1)
delta G9 (5)
dG9 (2)
MRQ-23 (1)
PRF1/2468 (1)
PRF1/2470 (2)
APC (3)
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Azide-free (2)
Biotin Conjugated (8)
BSA-free (2)
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Cy3 Conjugated (3)
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FITC Conjugated (7)
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PE Conjugated (4)
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RPE Conjugated (1)
Unconjugated (33)
aa120-353 (8)
aa32-316 (8)
aa40-355 (8)
aa146-195 (4)
C-Terminus (1)
aa175-555 (1)
aa461-555 (1)
aa9-214 (1)
No (67)
Yes (1)
Select
PRF1 / Perforin Rat anti-Mouse Monoclonal (CB5.4) Antibody
Mouse
ICC, IHC, IHC-Fr, IHC-P, IP, WB
Unconjugated
100 µg/$508
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded rat kidney using PRF1 antibody at dilution of 1:200 (x400 lens).
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PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
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PRF1 / Perforin Mouse anti-Human Monoclonal (delta G9) Antibody
Human
Flo, IHC, IHC-Fr
Unconjugated
100 µg/$446
PRF1 / Perforin Antibody - Flow cytometry of PRF1 / Perforin antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRF1 / Perforin Mouse anti-Human Monoclonal (Biotin) (delta G9) Antibody
Human
Flo, IHC, IHC-Fr
Biotin Conjugated
100 µg/$536
PRF1 / Perforin Antibody - Flow cytometry of PRF1 / Perforin antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRF1 / Perforin Mouse anti-Human Monoclonal (FITC) (delta G9) Antibody
Human
Flo, IHC, IHC-Fr
FITC Conjugated
120 Tests/$536
PRF1 / Perforin Antibody - Flow cytometry of PRF1 / Perforin antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PRF1 / Perforin Mouse anti-Human Monoclonal (RPE) (delta G9) Antibody
Human
Flo, IHC, IHC-Fr
RPE Conjugated
120 Tests/$561
PRF1 / Perforin Antibody - Western Blot analysis of PRF1 expression in transfected 293T cell line by PRF1 monoclonal antibody (M04), clone 3B4.Lane 1: PRF1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.
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PRF1 / Perforin Mouse anti-Human Monoclonal (aa461-555) (3B4) Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$423
PRF1 / Perforin Antibody - IHC of Perforin on FFPE Lymphoma tissue.
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PRF1 / Perforin Mouse anti-Human Monoclonal (5B10) Antibody
Human
IHC, IHC-Fr, IHC-P
Unconjugated
0.1 ml/$343
PRF1 / Perforin Antibody - IHC of Perforin on FFPE Lymphoma tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
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PRF1 / Perforin Mouse anti-Human Monoclonal (Ready-to-Use) (5B10) Antibody
Human
IHC-Fr, IHC-P
Unconjugated, Ready-to-Use
3 ml/$351
PRF1 / Perforin Antibody - Western Blot of human PBMC (Lane A) or PBMC stimulated with IL-2 for 3 days (Lane B) lysate; probed with 2 ug/mL purified antibody and anti-actin and revealed with HRP anti-rat IgG or anti-mouse IgG, respectively. Perforin is detected at ~65 kD and actin at ~42 kD. Actin was used a loading control to normalize the signal.
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PRF1 / Perforin Mouse anti-Human Monoclonal (BOR21) Antibody
Human
WB
Unconjugated
100 µg/$442
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PRF1 / Perforin Mouse anti-Human Monoclonal (dG9) Antibody
Bovine, Human
Flo, ICC, IHC-Fr, IHC-P, IP
Unconjugated
0.1 mg/$316
PRF1 / Perforin Antibody - Intracellular staining of perforin in human peripheral blood cells with anti-perforin (dG9) PE.
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PRF1 / Perforin Mouse anti-Human Monoclonal (PE) (dG9) Antibody
Bovine, Human
Flo, ICC, IHC-Fr, IHC-P, IP
PE Conjugated
100 Tests/$365
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PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
50 µl/$334; 100 µl/$397
PRF1 / Perforin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PRF1 / Perforin Rabbit anti-Rat Polyclonal (aa120-353) Antibody
Rat
IHC, IHC-P, WB
Unconjugated
50 µl/$460
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PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
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PRF1 / Perforin Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
PRF1 / Perforin Mouse anti-Human Monoclonal (Biotin) Antibody
Human
Flo
Biotin Conjugated
100 µg/$763
PRF1 / Perforin Antibody - Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. Perforin 1 antibody. Perforin antibody diluted at 1:1000.
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PRF1 / Perforin Rabbit anti-Human Polyclonal (aa9-214) Antibody
Human
WB
Unconjugated
100 µl/$450
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded Mouse spleen using Perforin Polyclonal Antibody at dilution of 1:100 (40x lens).
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PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
PRF1 / Perforin Antibody - Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
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PRF1 / Perforin Mouse anti-Human Monoclonal (Concentrated) (MRQ-23) Antibody
Human
IHC, IHC-Fr, IHC-P
Unconjugated, Concentrated
1 ml/$1,216
PRF1 / Perforin Antibody - 1:100 staining human urothelial carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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PRF1 / Perforin Mouse anti-Human Monoclonal (C-Terminus) Antibody
Human
IHC, IHC-P
Unconjugated
500 µl/$939
Select
PRF1 / Perforin Mouse anti-Human Monoclonal Antibody
Human
Flo, IP
Unconjugated
100 µg/$626
Select
PRF1 / Perforin Mouse anti-Human Monoclonal (delta G9) Antibody
Human
Flo, IP
Unconjugated
100 µg/$760
Viewing 1-25 of 68 product results


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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).