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Antibodies > XRCC3 > LS-C312851
 
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Catalog Number Size Price
LS-C312851-10 10 µg $318 
LS-C312851-100 100 µg (0.5 mg/ml) $470 
XRCC3 Antibody - IHC analysis of XRCC3 using anti-XRCC3 antibody. XRCC3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-XRCC3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
XRCC3 Antibody - WB of XRCC3 antibody. All lanes: Anti-XRCC3 at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: HT1080 Whole Cell Lysate at 40ug. Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: MM231 Whole Cell Lysate at 40ug. Predicted bind size: 38KD. Observed bind size: 38KD.
XRCC3 Antibody - Western blot analysis of -XRCC3 using anti--XRCC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Mouse Brain Tissue Lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--XRCC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for -XRCC3 at approximately 38KD. The expected band size for -XRCC3 is at 38KD.
XRCC3 Antibody - IHC analysis of XRCC3 using anti-XRCC3 antibody. XRCC3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-XRCC3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
XRCC3 Antibody - WB of XRCC3 antibody. All lanes: Anti-XRCC3 at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: HT1080 Whole Cell Lysate at 40ug. Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: MM231 Whole Cell Lysate at 40ug. Predicted bind size: 38KD. Observed bind size: 38KD.
XRCC3 Antibody - Western blot analysis of -XRCC3 using anti--XRCC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Mouse Brain Tissue Lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--XRCC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for -XRCC3 at approximately 38KD. The expected band size for -XRCC3 is at 38KD.
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Polyclonal Rabbit anti‑Mouse XRCC3 Antibody (aa290‑305, IHC, WB) LS‑C312851

Polyclonal Rabbit anti‑Mouse XRCC3 Antibody (aa290‑305, IHC, WB) LS‑C312851

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Note: This antibody replaces LS-C389415
Antibody:
XRCC3 Rabbit anti-Mouse Polyclonal (aa290-305) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312851-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
XRCC3 Rabbit anti-Mouse Polyclonal (aa290-305) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
XRCC3 antibody LS-C312851 is an unconjugated rabbit polyclonal antibody to XRCC3 (aa290-305) from mouse. It is reactive with mouse and rat. Validated for IHC and WB.
Target
Mouse XRCC3
Synonyms
XRCC3 | DNA repair protein XRCC3 | CMM6
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of mouse XRCC3(290-305 aa NQLLMRLMVDRTHEDD), identical to the related rat sequence.
Epitope
aa290-305
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About XRCC3
O43542 NM_005432 NP_005423.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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