Human WNK4

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

A 13 AA peptide sequence, mapping at the middle (around ~650 AA) region of human WNK-4 (1) synthesized, coupled to KLH.

Internal

Peptide sequences from other species are not yet available. No significant sequence homology of is seen with other WNKs or proteins. Antibody reactivity in various species is not established. The control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity.

• Western blot (1:1000 - 1:5000)
• ELISA (1:10000 - 1:100000)

Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. WNK4 is ~135/155kD in kidney. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.

PBS, pH 7.4, 0.1% BSA

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96J92 NCBI: NM_032387 NP_115763.2