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Catalog Number Size Price
LS-C313035-10 10 µg $318 
LS-C313035-100 100 µg (0.5 mg/ml) $470 
p66 / SHC Antibody - p66 / SHC antibody. IHC(F): Rat Intestine Tissue.
p66 / SHC Antibody - p66 / SHC antibody. IHC(P): Human Lung Cancer Tissue.
p66 / SHC Antibody - p66 / SHC antibody. IHC(P): Rat Brain Tissue.
p66 / SHC Antibody - Anti-SHC antibody, ICCICC: A549 Cell
p66 / SHC Antibody - p66 / SHC antibody. IHC(F): Rat Brain Tissue.
p66 / SHC Antibody - Anti-SHC antibody, Western blottingRecombinant Protein Detection Source: E. coli derived -recombinant human SHC1, 35. 0KD(162aa tag+D424-P578)Lane 1: Recombinant Human SHC1 Proteins 10ng Lane 2: Recombinant Human SHC1 Proteins 5ng Lane 3: Recombinant Human SHC1 Proteins 2. 5ng
p66 / SHC Antibody - Western blot analysis of SHC using anti-SHC antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human HepG2 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: rat C6 whole cell lysate, Lane 6: mouse thymus tissue lysate, Lane 7: mouse RAW246. 7 whole cell lysate, Lane 8: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
p66 / SHC Antibody - WB of p66 / SHC antibody. Lane 1: Recombinant Human SHC1 Protein 10ng. Lane 2: Recombinant Human SHC1 Protein 5ng. Lane 3: Recombinant Human SHC1 Protein 2.5ng.
p66 / SHC Antibody - WB of p66 / SHC antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: A549 Cell Lysate. Lane 3: A431 Cell Lysate. Lane 4: 293T Cell Lysate. Lane 5: HELA Cell Lysate. Lane 6: JURKAT Cell Lysate..
p66 / SHC Antibody - p66 / SHC antibody. IHC(F): Rat Intestine Tissue.
p66 / SHC Antibody - p66 / SHC antibody. IHC(P): Human Lung Cancer Tissue.
p66 / SHC Antibody - p66 / SHC antibody. IHC(P): Rat Brain Tissue.
p66 / SHC Antibody - Anti-SHC antibody, ICCICC: A549 Cell
p66 / SHC Antibody - p66 / SHC antibody. IHC(F): Rat Brain Tissue.
p66 / SHC Antibody - Anti-SHC antibody, Western blottingRecombinant Protein Detection Source: E. coli derived -recombinant human SHC1, 35. 0KD(162aa tag+D424-P578)Lane 1: Recombinant Human SHC1 Proteins 10ng Lane 2: Recombinant Human SHC1 Proteins 5ng Lane 3: Recombinant Human SHC1 Proteins 2. 5ng
p66 / SHC Antibody - Western blot analysis of SHC using anti-SHC antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human HepG2 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: rat C6 whole cell lysate, Lane 6: mouse thymus tissue lysate, Lane 7: mouse RAW246. 7 whole cell lysate, Lane 8: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
p66 / SHC Antibody - WB of p66 / SHC antibody. Lane 1: Recombinant Human SHC1 Protein 10ng. Lane 2: Recombinant Human SHC1 Protein 5ng. Lane 3: Recombinant Human SHC1 Protein 2.5ng.
p66 / SHC Antibody - WB of p66 / SHC antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: A549 Cell Lysate. Lane 3: A431 Cell Lysate. Lane 4: 293T Cell Lysate. Lane 5: HELA Cell Lysate. Lane 6: JURKAT Cell Lysate..
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Polyclonal Rabbit anti‑Human p66 / SHC Antibody (aa424‑440, IHC, WB) LS‑C313035

Polyclonal Rabbit anti‑Human p66 / SHC Antibody (aa424‑440, IHC, WB) LS‑C313035

Note: This antibody replaces LS-C389252
Antibody:
p66 / SHC Rabbit anti-Human Polyclonal (aa424-440) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313035-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
p66 / SHC Rabbit anti-Human Polyclonal (aa424-440) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SHC antibody LS-C313035 is an unconjugated rabbit polyclonal antibody to SHC (p66) (aa424-440) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human p66 / SHC
Synonyms
SHC1 | p46 shc | p52 shc | SH2 domain protein C1 | p66 | SHCA | SHC | SHC-transforming protein 1 | SHC-transforming protein 3 | SHC-transforming protein A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human SHC(424-440 aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids.
Epitope
aa424-440
Specificity
Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About p66 / SHC
P29353 NM_003029 NP_003020.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/15/2024