Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 4D11, IHC, WB) LS‑C204544
Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 4D11, IHC, WB) LS‑C204544
Note: This antibody replaces LS-C180217, LS-B9428
Antibody:
NES / Nestin Mouse anti-Human Monoclonal (4D11) Antibody
Application:
IHC, ICC, WB
Format:
Unconjugated, Unmodified
Overview
Antibody:
NES / Nestin Mouse anti-Human Monoclonal (4D11) Antibody
Application:
IHC, ICC, WB
Format:
Unconjugated, Unmodified
Specifications
Description
Nestin antibody LS-C204544 is an unconjugated mouse monoclonal antibody to human Nestin (NES). Validated for ICC, IHC and WB.
Target
Human NES / Nestin
Synonyms
NES | Nestin | Nbla00170
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Recombinant human Nestin expressed in bacteria and highly purified. The specific region was amino acids 317 to 630 of the human protein. This is a region which is 55% identical to rat Nestin and 57% identical to mouse Nestin.
Applications
- IHC (1:500)
- ICC (1:500)
- Western blot
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
For immunocytochemistry on cells in tissue culture or in tissue sections, try this antibody at about between about 1:500 using fluorescent secondary antibodies. For immunoblotting 1:5000 is recommended. Expect to see a band at ~240kDa and another much weaker at ~90kDa, apparently a breakdown product of the ~240kDa band.
Presentation
Ascites fluid.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Rat, Mouse
Applications:
IHC, IHC - Frozen, ICC, Western blot
Species:
Rat
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/21/2024