Target
Human LCN2 / Lipocalin 2 / NGAL
Synonyms
LCN2 | 24p3 | HNL | Lipocalin 2 | Lipocalin-2 | MSFI | Siderocalin LCN2 | NGAL | p25 | Lipocalin 2 (oncogene 24p3) | Oncogene 24p3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Immunogen
KLH-conjugated synthetic peptide of human NGAL
Specificity
Recognizes endogenous levels of NGAL protein.
Applications
- ICC
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
Usage
Western blot analysis of NGAL expression in SW480 (A), A549 (B) whole cell lysates. Immunofluorescent analysis of NGAL staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LCN2 / Lipocalin 2 / NGAL