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Catalog Number Size Price
LS-C392265-100 100 µg (1 mg/ml) $574 
KRT6 / CK6 / Cytokeratin 6 Antibody - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269).  This image was taken for the unmodified form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human KRT6 / CK6 / Cytokeratin 6 Antibody (clone SPM269, Azide‑free,BSA‑free, aa545‑555, IHC, IF) LS‑C392265

Monoclonal Mouse anti‑Human KRT6 / CK6 / Cytokeratin 6 Antibody (clone SPM269, Azide‑free,BSA‑free, aa545‑555, IHC, IF) LS‑C392265

Antibody:
KRT6 / CK6 / Cytokeratin 6 Mouse anti-Human Monoclonal (aa545-555) (Azide-free, BSA-free) (SPM269) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Horse, Sheep
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Price
Catalog Number
$574
LS-C392265-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
KRT6 / CK6 / Cytokeratin 6 Mouse anti-Human Monoclonal (aa545-555) (Azide-free, BSA-free) (SPM269) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Horse, Sheep
Format:
Unconjugated, Azide-free, BSA-free
Other formats:

Specifications

Description
Cytokeratin 6 antibody LS-C392265 is an unconjugated mouse monoclonal antibody to Cytokeratin 6 (KRT6 / CK6) (aa545-555) from human. It is reactive with human, horse and sheep. Validated for Flow, IF and IHC.
Target
Human KRT6 / CK6 / Cytokeratin 6
Host
Mouse
Reactivity
Human, Horse, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
SPM269
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free. Also available Unmodified.
Immunogen
Synthetic peptide from human keratin 6 (GSSTIKYTTTS, aa545-555 of UniProt P48668). Percent identity by BLAST analysis: Human, Sheep, Horse (100%); Mouse, Rat, Ferret, Cat, Bat, Hamster, Elephant, Dog (91%); Bovine, Pig, Opossum (82%).
Epitope
aa545-555
Specificity
Human KRT6 / CK6 / Cytokeratin 6
Applications
  • IHC
  • IHC - Paraffin (0.1 - 0.2 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. The optimal dilution of the Cytokeratin 6 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
1X PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024