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Catalog Number Size Price
LS-C757834-200 200 µl (0.25 mg/ml) $393 
KL / Klotho Antibody - Anti-KL Antibody (Center) at 1:2000 dilution + Human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 116 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
KL / Klotho Antibody - Overlay histogram showing HepG2 cells stained with KL Antibody (Center) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (KL Antibody (Center), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
KL / Klotho Antibody - Anti-KL Antibody (Center) at 1:2000 dilution + Human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 116 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
KL / Klotho Antibody - Overlay histogram showing HepG2 cells stained with KL Antibody (Center) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (KL Antibody (Center), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human KL / Klotho Antibody (Internal, WB) LS‑C757834

Polyclonal Rabbit anti‑Human KL / Klotho Antibody (Internal, WB) LS‑C757834

Antibody:
KL / Klotho Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757834-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
KL / Klotho Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Klotho antibody LS-C757834 is an unconjugated rabbit polyclonal antibody to human Klotho (KL) (Internal). Validated for ELISA, Flow and WB.
Target
Human KL / Klotho
Synonyms
KL | Klotho
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This KL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 331-365 amino acids from the Central region of human KL.
Epitope
Internal
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 116.2 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KL / Klotho
Q9UEF7 NM_004795 NP_004786.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2024