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Catalog Number Size Price
LS-B17001-50 50 µg (0.5 mg/ml) $460 
HSPA8 / HSC70 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA8 / HSC70 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA8 / HSC70 Antibody - HSPA8 / HSC70 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HSPA8 / HSC70 Antibody - HSPA8 / HSC70 antibody (0.03µg/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
HSPA8 / HSC70 Antibody - HSPA8 antibody (2 ug/ml) staining of A431 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HSPA8 / HSC70 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA8 / HSC70 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA8 / HSC70 Antibody - HSPA8 / HSC70 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HSPA8 / HSC70 Antibody - HSPA8 / HSC70 antibody (0.03µg/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
HSPA8 / HSC70 Antibody - HSPA8 antibody (2 ug/ml) staining of A431 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human HSPA8 / HSC70 Antibody (Isoform 1, IHC, IF, WB) LS‑B17001

IHC‑plus™ Polyclonal Goat anti‑Human HSPA8 / HSC70 Antibody (Isoform 1, IHC, IF, WB) LS‑B17001

Note: This antibody replaces LS-C34676
Antibody:
HSPA8 / HSC70 Goat anti-Human Polyclonal (Isoform 1) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B17001-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPA8 / HSC70 Goat anti-Human Polyclonal (Isoform 1) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
HSC70 antibody LS-B17001 is an unconjugated goat polyclonal antibody to HSC70 (HSPA8) (Isoform 1) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPA8 / HSC70
Synonyms
HSPA8 | Heat shock 70 kDa protein 8 | Heat shock 70kd protein 10 | Heat shock 70kD protein 8 | Heat shock cognate protein 54 | HSP71 | HSPA10 | HSC71 | HSP73 | LPS-associated protein 1 | Heat shock 70kDa protein 8 | NIP71 | HSC54 | HSC70 | LAP1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.
Epitope
Isoform 1
Specificity
Human HSPA8 / HSC70. This antibody is expected to recognise isoform 1 (NP_006588.1) only.
Applications
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 75 kDa band observed in lysates of cell line A431 (calculated MW of 70.9 kDa according to NP_006588.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA8 / HSC70
P11142 NM_006597 NP_006588.1

Validation

HSPA8 / HSC70 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA8 / HSC70 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ HSPA8 / HSC70 Antibody (Isoform 1) for IHC, IF/Immunofluorescence, WB/Western LS-B17001 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024