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Catalog Number Size Price
LS-B14963-50 50 µg (0.8 mg/ml) $460 
CD3E Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD3E Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD3E Antibody - Immunofluorescence staining of PC3 cells with CD3E Antibody at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CD3E Antibody - Western blot All lanes: CD3E antibody at 1.5µg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
CD3E Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD3E Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD3E Antibody - Immunofluorescence staining of PC3 cells with CD3E Antibody at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CD3E Antibody - Western blot All lanes: CD3E antibody at 1.5µg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
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IHC‑plus™ Polyclonal Rabbit anti‑Human CD3E Antibody (aa 23‑207, IHC, WB) LS‑B14963

IHC‑plus™ Polyclonal Rabbit anti‑Human CD3E Antibody (aa 23‑207, IHC, WB) LS‑B14963

Note: This antibody replaces LS-C211915
Antibody:
CD3E Rabbit anti-Human Polyclonal (aa 23-207) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-B14963-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD3E Rabbit anti-Human Polyclonal (aa 23-207) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD3E antibody LS-B14963 is an unconjugated rabbit polyclonal antibody to human CD3E (aa 23-207). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human T-cell surface glycoprotein CD3 epsilon chain protein.
Epitope
aa 23-207
Specificity
Human CD3E
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD3E

Validation

CD3E Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

CD3E Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CD3E Antibody (aa 23-207) for IHC, WB/Western, ELISA LS-B14963 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024