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Catalog Number Size Price
LS-B13294-50 50 µl $460 
ATP1A1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
ATP1A1 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
ATP1A1 Antibody - Western blot analysis of extracts of LO2 cells, using ATP1A1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
ATP1A1 Antibody - Western blot analysis of extracts of A549 cell and 293 cell line, using ATP1A1 antibody.
ATP1A1 Antibody - Immunoprecipitation analysis of 200ug extracts of LO2 cells, using 3 ug ATP1A1 antibody. Western blot was performed from the immunoprecipitate using ATP1A1 antibodyat a dilition of 1:1000.
ATP1A1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
ATP1A1 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
ATP1A1 Antibody - Western blot analysis of extracts of LO2 cells, using ATP1A1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
ATP1A1 Antibody - Western blot analysis of extracts of A549 cell and 293 cell line, using ATP1A1 antibody.
ATP1A1 Antibody - Immunoprecipitation analysis of 200ug extracts of LO2 cells, using 3 ug ATP1A1 antibody. Western blot was performed from the immunoprecipitate using ATP1A1 antibodyat a dilition of 1:1000.
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IHC‑plus™ Polyclonal Mouse anti‑Human ATP1A1 Antibody (IHC, IF, WB) LS‑B13294

IHC‑plus™ Polyclonal Mouse anti‑Human ATP1A1 Antibody (IHC, IF, WB) LS‑B13294

Note: This antibody replaces LS-C331040
Antibody:
ATP1A1 Mouse anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B13294-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATP1A1 Mouse anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ATP1A1 antibody LS-B13294 is an unconjugated mouse polyclonal antibody to human ATP1A1. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ATP1A1
Synonyms
ATP1A1 | Na+, K+ ATPase alpha subunit | Na,K-ATPase alpha-1 subunit | Na+/K+ ATPase 1 | Sodium pump 1 | Sodium pump subunit alpha-1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A1 (NP_000692.2). RVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLI
Specificity
Human ATP1A1
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:20 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 74kDa/109kDa/112kDa/113kDa, while the observed MW by Western blot was 113kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATP1A1
P05023 NM_000701 NP_000692.2

Validation

ATP1A1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

ATP1A1 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ ATP1A1 Antibody for IHC, IF/Immunofluorescence, WB/Western, IP LS-B13294 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024