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Catalog Number Size Price
LS-B15324-50 50 µl $460 
APG5 / ATG5 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
APG5 / ATG5 Antibody - Immunohistochemistry of paraffin-embedded human prostate, with a concentration of 1:100.
APG5 / ATG5 Antibody - Immunofluorescence analysis of HeLa cells using ATG5 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
APG5 / ATG5 Antibody - Western blot analysis of extracts of various cell lines, using ATG5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
APG5 / ATG5 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
APG5 / ATG5 Antibody - Immunohistochemistry of paraffin-embedded human prostate, with a concentration of 1:100.
APG5 / ATG5 Antibody - Immunofluorescence analysis of HeLa cells using ATG5 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
APG5 / ATG5 Antibody - Western blot analysis of extracts of various cell lines, using ATG5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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IHC‑plus™ Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (IHC, IF, WB) LS‑B15324

IHC‑plus™ Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (IHC, IF, WB) LS‑B15324

Note: This antibody replaces LS-C330829
Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B15324-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ATG5 antibody LS-B15324 is an unconjugated rabbit polyclonal antibody to ATG5 (APG5) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human APG5 / ATG5
Synonyms
ATG5 | APG5 | APG5-LIKE | APG5L | Apoptosis specific protein | Apoptosis-specific protein | Autophagy related 5 | HAPG5 | ASP | Autophagy protein 5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1). MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD
Specificity
Human APG5 / ATG5
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 22kDa/32kDa, while the observed MW by Western blot was 58kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG5 / ATG5
Q9H1Y0 NM_004849 NP_004840.1

Validation

APG5 / ATG5 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.

LSBio Ratings

IHC-plus™ APG5 / ATG5 Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B15324 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024